PubMed Journals: Cell
Source: PMID: 8548797
⇦ ⇨ Cell. 1995 Dec 29;83(7):1091-100.
Virus induction of human IFN beta gene expression
requires the assembly of an enhanceosome.
Thanos D(1), Maniatis T.
(1) Department of Molecular and Cellular
Biology, Harvard University, Cambridge,
Massachusetts 02138, USA.
We present evidence that transcriptional
activation of the human interferon-beta
(IFN beta) gene requires the assembly of
a higher order transcription enhancer complex
(enhanceosome). This multicomponent complex
includes at least three distinct transcription
factors and the high mobility group protein
HMG I(Y). Both the in vitro assembly and
in vivo transcriptional activity of this
complex require a precise helical relationship
between individual transcription factor-binding
sites. In addition, HMG I(Y), which binds
specifically to three sites within the enhancer,
promotes cooperative binding of transcriptional
factors in vitro and is required for transcriptional
synergy between these factors in vivo. Thus,
HMG I(Y) plays an essential role in the
assembly and function of the IFN beta gene
PMID: 8548797 [Indexed for MEDLINE]