PubMed Journals: J Biol Chem
Source: PMID: 8407908
⇦ ⇨ J Biol Chem. 1993 Oct 5;268(28):20801-7.
Cloning and characterization of the gene
encoding the human platelet glycoprotein
V. A member of the leucine-rich glycoprotein
family cleaved during thrombin-induced platelet
Lanza F(1), Morales M, de La Salle C, Cazenave
JP, Clemetson KJ, Shimomura T, Phillips
(1) Centre Régional de Transfusion Sanguine,
Institut National de la Santé et de la Recherche
Médicale Unité 311, Strasbourg, France.
Glycoprotein V (GPV) is a major platelet
membrane 82-kDa glycoprotein, missing in
the Bernard-Soulier syndrome, that is cleaved
when platelets are treated with thrombin.
We report the cloning and sequencing of
the GPV cDNA and gene obtained by a combination
of polymerase chain reaction amplification
of platelet mRNA and genomic library screening.
The single-copy gene for GPV is contained
within 6.5 kilobase pairs (kb) of genomic
sequence and has a simple structure with
a single intron of 958 base pairs in the
5'-untranslated sequence; the coding sequence
is contained within a single exon. The promoter
region contains a canonical TATA box, and
putative GATA, Ets-1, and Sp1 cis-acting
elements. Reverse transcription-polymerase
chain reaction analysis on RNAs from cells
of different hematopoietic origins revealed
that GPV was specifically transcribed from
platelets and from cells of the megakaryocytic
lineage (megakaryocytes, HEL cells). A single
transcript of 4.5 kb for GPV was detected
in human platelets by Northern blot analysis.
The entire amino acid sequence of GPV was
deduced from the cDNA and genomic sequences.
Mature GPV was composed of 544 amino acids
which contained a single transmembrane domain,
a short cytoplasmic domain (16 residues),
and a large extracellular domain with 8
potential N-glycosylation sites. Analysis
of the extracellular domain revealed the
presence of 15 tandem Leu-rich repeats of
24 amino acids with homology to GPIb alpha
and identified a cleavage site for thrombin
near the COOH terminus with similarity to
the A alpha chain of fibrinogen, but no
hirudin-like sequence was found.
PMID: 8407908 [Indexed for MEDLINE]