PubMed Journals: Oxid Med Cell Longev
Source: PMID: 31223425⇦⇨ Oxid Med Cell Longev. 2019 May 19;2019:7071536.
⇩ doi: 10.1155/2019/7071536. eCollection 2019.
Improved Wound Healing of Airway Epithelial
Cells Is Mediated by Cold Atmospheric Plasma:
A Time Course-Related Proteome Analysis.
Scharf C(1), Eymann C(1), Emicke P(1), Bernhardt
J(2), Wilhelm M(1), Görries F(1), Winter
J(3), von Woedtke T(3)(4), Darm K(1), Daeschlein
G(5), Steil L(6), Hosemann W(1), Beule A(1)(7).
(1) Department of Otorhinolaryngology, Head
and Neck Surgery, University Medicine Greifswald,
(2) Institute for Microbiology, University
of Greifswald, Germany.
(3) Leibniz Institute for Plasma Science
and Technology (INP), Greifswald, Germany.
(4) Department of Hygiene and Environmental
Medicine, University Medicine Greifswald,
(5) Department of Dermatology, University
Medicine Greifswald, Greifswald, Germany.
(6) Interfaculty Institute of Genetics and
Functional Genomics, University of Greifswald,
(7) Department of Otorhinolaryngology, University
Hospital Münster, Münster, Germany.
The promising potential of cold atmospheric
plasma (CAP) treatment as a new therapeutic
option in the field of medicine, particularly
in Otorhinolaryngology and Respiratory medicine,
demands primarily the assessment of potential
risks and the prevention of any direct and
future cell damages. Consequently, the application
of a special intensity of CAP that is well
tolerated by cells and tissues is of particular
interest. Although improvement of wound
healing by CAP treatment has been described,
the underlying mechanisms and the molecular
influences on human tissues are so far only
partially characterized. In this study,
human S9 bronchial epithelial cells were
treated with cold plasma of atmospheric
pressure plasma jet that was previously
proven to accelerate the wound healing in
a clinically relevant extent. We studied the
detailed cellular adaptation reactions
for a specified plasma intensity by time-resolved
comparative proteome analyses of plasma
treated vs. nontreated cells to elucidate the
mechanisms of the observed improved wound
healing and to define potential biomarkers
and networks for the evaluation of plasma
effects on human epithelial cells. K-means
cluster analysis and time-related analysis of fold-change
factors indicated concordantly clear differences
between the short-term (up to 1 h) and long-term
(24-72 h) adaptation reactions. Thus, the
induction of Nrf2-mediated oxidative and
endoplasmic reticulum stress response,
PPAR-alpha/RXR activation as well as production
of peroxisomes, and prevention of apoptosis already
during the first hour after CAP treatment
are important cell strategies to overcome
oxidative stress and to protect and maintain
cell integrity and especially microtubule
dynamics. After resolving of stress, when
stress adaptation was accomplished, the
cells seem to start again with proliferation
and cellular assembly and organization.
The observed strategies and identification
of marker proteins might explain the accelerated
wound healing induced by CAP, and these
indicators might be subsequently used for
risk assessment and quality management of
application of nonthermal plasma sources
in clinical settings.
DOI: 10.1155/2019/7071536PMCID: PMC6541959PMID: 31223425⇧TweetPrint