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			PubMed Journals: Oxid Med Cell Longev

  Source:		PMID: 31223425


    		Oxid Med Cell Longev. 2019 May 19;2019:7071536.
     		doi: 10.1155/2019/7071536. eCollection 2019.

			Improved Wound Healing of Airway Epithelial
			Cells Is Mediated by Cold Atmospheric Plasma:
			A Time Course-Related Proteome Analysis.

			Scharf C(1), Eymann C(1), Emicke P(1), Bernhardt
			J(2), Wilhelm M(1), Görries F(1), Winter
			J(3), von Woedtke T(3)(4), Darm K(1), Daeschlein
			G(5), Steil L(6), Hosemann W(1), Beule A(1)(7).

			Author Information
			(1) Department of Otorhinolaryngology, Head
			and Neck Surgery, University Medicine Greifswald,
			Germany.
			(2) Institute for Microbiology, University
			of Greifswald, Germany.
			(3) Leibniz Institute for Plasma Science
			and Technology (INP), Greifswald, Germany.
			(4) Department of Hygiene and Environmental
			Medicine, University Medicine Greifswald,
			Greifswald, Germany.
			(5) Department of Dermatology, University
			Medicine Greifswald, Greifswald, Germany.
			(6) Interfaculty Institute of Genetics and
			Functional Genomics, University of Greifswald,
			Germany.
			(7) Department of Otorhinolaryngology, University
			Hospital Münster, Münster, Germany.

			The promising potential of cold atmospheric
			plasma (CAP) treatment as a new therapeutic
			option in the field of medicine, particularly
			in Otorhinolaryngology and Respiratory medicine,
			demands primarily the assessment of potential
			risks and the prevention of any direct and
			future cell damages. Consequently, the application
			of a special intensity of CAP that is well
			tolerated by cells and tissues is of particular
			interest. Although improvement of wound
			healing by CAP treatment has been described,
			the underlying mechanisms and the molecular
			influences on human tissues are so far only
			partially characterized. In this study,
			human S9 bronchial epithelial cells were
			treated with cold plasma of atmospheric
			pressure plasma jet that was previously
			proven to accelerate the wound healing in
			a clinically relevant extent. We studied the
			detailed cellular adaptation reactions
			for a specified plasma intensity by time-resolved
			comparative proteome analyses of plasma
			treated vs. nontreated cells to elucidate the
			mechanisms of the observed improved wound
			healing and to define potential biomarkers
			and networks for the evaluation of plasma
			effects on human epithelial cells. K-means
			cluster analysis and time-related analysis of fold-change
			factors indicated concordantly clear differences
			between the short-term (up to 1 h) and long-term
			(24-72 h) adaptation reactions. Thus, the
			induction of Nrf2-mediated oxidative and
			endoplasmic reticulum stress response,
			PPAR-alpha/RXR activation as well as production
			of peroxisomes, and prevention of apoptosis already
			during the first hour after CAP treatment
			are important cell strategies to overcome
			oxidative stress and to protect and maintain
			cell integrity and especially microtubule
			dynamics. After resolving of stress, when
			stress adaptation was accomplished, the
			cells seem to start again with proliferation
			and cellular assembly and organization.
			The observed strategies and identification
			of marker proteins might explain the accelerated
			wound healing induced by CAP, and these
			indicators might be subsequently used for
			risk assessment and quality management of
			application of nonthermal plasma sources
			in clinical settings.

			DOI: 10.1155/2019/7071536 PMCID: PMC6541959
			PMID: 31223425

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