*nlm.life
			PubMed Journals: BMC Vet Res

  Source:		PMID: 30971240


    		BMC Vet Res. 2019 Apr 11;15(1):111. doi:
     		10.1186/s12917-019-1862-4.

			Analysis of long non-coding RNAs in neonatal
			piglets at different stages of porcine
			deltacoronavirus infection.

			Tang X(1)(2), Lan T(1)(2), Wu R(1)(2), Zhou
			Z(1)(2), Chen Y(1)(2), Sun Y(1)(2), Zheng
			Y(1)(2), Ma J(3)(4).

			Author Information
			(1) College of Animal Science, South
			China Agricultural University, Tianhe District,
			Wushan Road 483, Guangzhou, 510642, China.
			(2) Key Laboratory of Animal Health Aquaculture
			and Environmental Control, Guangzhou, Guangdong,
			China.
			(3) College of Animal Science, South China
			Agricultural University, Tianhe District,
			Wushan Road 483, Guangzhou, 510642, China.
			majy2400@scau.edu.cn.
			(4) Key Laboratory of Animal Health Aquaculture
			and Environmental Control, Guangzhou, Guangdong,
			China. majy2400@scau.edu.cn.

			BACKGROUND: PDCoV (Porcine Deltacoronavirus)
			is a novel porcine coronavirus that causes
			intestinal necrosis of piglets, thinning
			of the intestinal wall and severe villus
			atrophy in the small intestine. PDCoV is
			a highly contagious infectious disease characterized
			by diarrhea, dehydration and vomiting. It
			has been reported that lncRNA has a significant
			effect on viral replication and increased
			or decreased virulence. At present, there
			is almost no research on lncRNA related
			to PDCoV infection. With the development
			of the research, a large number of lncRNAs
			related to PDCoV infection have been discovered.
			Identifying the role of these lncRNAs in
			the infection process facilitates the screening
			of diagnostically significant biomarkers.
			RESULTS: Using high throughput sequencing
			to screen differentially expressed long
			non-coding RNA (lncRNA) during PDCoV infection,
			we identified 99, 41 and 33 differentially
			expressed lncRNAs in the early, middle and
			late stages of infection, respectively.
			These lncRNAs were involved in glycolysis
			/ gluconeogenesis, histidine metabolism
			and pentose and Chloroalkane and chloroalkene
			degradation pathway. We obtained expression
			data of miRNAs, lncRNAs and mRNAs during
			PDCoV infection and constructed and investigated
			an interaction network. The qRT-PCR validation
			results of 6 differentially expressed lncRNAs
			were consistent with RNA-Seq results. CONCLUSIONS:
			This study is the first to examine differentially
			expressed lncRNAs after PDCoV infection
			of piglets. These results can provide new
			insights into PDCoV infection and antiviral
			strategies.

			DOI: 10.1186/s12917-019-1862-4 PMCID: PMC6458635
			PMID: 30971240 [Indexed for MEDLINE]

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