PubMed Journals: Virol J
Source: PMID: 16571117
⇦ ⇨ Virol J. 2006 Mar 29;3:17.
Inhibition of cytokine gene expression and
induction of chemokine genes in non-lymphatic
cells infected with SARS coronavirus.
Spiegel M(1), Weber F.
(1) Abteilung Virologie, Institut für Medizinische
Mikrobiologie und Hygiene, Universität,
Freiburg, D-79008 Freiburg, Germany.
BACKGROUND: SARS coronavirus (SARS-CoV)
is the etiologic agent of the severe acute
respiratory syndrome. SARS-CoV mainly infects
tissues of non-lymphatic origin, and the
cytokine profile of those cells can determine
the course of disease. Here, we investigated
the cytokine response of two human non-lymphatic
cell lines, Caco-2 and HEK 293, which are
fully permissive for SARS-CoV. RESULTS:
A comparison with established cytokine-inducing
viruses revealed that SARS-CoV only weakly
triggered a cytokine response. In particular,
SARS-CoV did not activate significant transcription
of the interferons IFN-alpha, IFN-beta,
IFN-lambda1, IFN-lambda2/3, as well as of
the interferon-induced antiviral genes ISG56
and MxA, the chemokine RANTES and the interleukine
IL-6. Interestingly, however, SARS-CoV strongly
induced the chemokines IP-10 and IL-8 in
the colon carcinoma cell line Caco-2, but
not in the embryonic kidney cell line 293.
CONCLUSION: Our data indicate that SARS-CoV suppresses
the antiviral cytokine system of non-immune
cells to a large extent, thus buying time
for dissemination in the host. However,
synthesis of IP-10 and IL-8, which are established
markers for acute-stage SARS, escapes the
virus-induced silencing at least in some
cell types. Therefore, the progressive infiltration
of immune cells into the infected lungs
observed in SARS patients could be due to
the production of these chemokines by the
infected tissue cells.
DOI: 10.1186/1743-422X-3-17 PMCID: PMC1444920
PMID: 16571117 [Indexed for MEDLINE]