PubMed Journals: J Virol

  Source:		PMID: 16306622
  Download:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1316022/pdf/1443-05.pdf

    		J Virol. 2005 Dec;79(24):15511-24.
			Severe acute respiratory syndrome coronavirus
			infection of human ciliated airway epithelia:
			role of ciliated cells in viral spread in
			the conducting airways of the lungs.

			Sims AC(1), Baric RS, Yount B, Burkett SE,
			Collins PL, Pickles RJ.

			Author Information
			(1) Department of Epidemiology, University
			of North Carolina at Chapel Hill, 2107
			McGavran-Greenberg Hall, CB 7435, Chapel
			Hill, NC 27599-7435, USA. sims0018@email.unc.edu

			Severe acute respiratory syndrome coronavirus
			(SARS-CoV) emerged in 2002 as an important cause
			of severe lower respiratory tract infection
			in humans, and in vitro models of the lung
			are needed to elucidate cellular targets
			and the consequences of viral infection.
			The SARS-CoV receptor, human angiotensin 1-converting enzyme
			2 (hACE2), was detected in ciliated airway epithelial
			cells of human airway tissues derived from
			nasal or tracheobronchial regions, suggesting
			that SARS-CoV may infect the proximal airways.
			To assess infectivity in an in vitro model
			of human ciliated airway epithelia (HAE)
			derived from nasal and tracheobronchial
			airway regions, we generated recombinant
			SARS-CoV by deletion of open reading frame
			7a/7b (ORF7a/7b) and insertion of the green
			fluorescent protein (GFP), resulting in
			SARS-CoV GFP. SARS-CoV GFP replicated to
			titers similar to those of wild-type viruses
			in cell lines. SARS-CoV specifically infected
			HAE via the apical surface and replicated
			to titers of 10(7) PFU/ml by 48 h postinfection.
			Polyclonal antisera directed against hACE2
			blocked virus infection and replication,
			suggesting that hACE2 is the primary receptor
			for SARS-CoV infection of HAE. SARS-CoV
			structural proteins and virions localized
			to ciliated epithelial cells. Infection
			was highly cytolytic, as infected ciliated
			cells were necrotic and shed over time onto
			the luminal surface of the epithelium. SARS-CoV
			GFP also replicated to a lesser extent in
			ciliated cell cultures derived from hamster
			or rhesus monkey airways. Efficient SARS-CoV
			infection of ciliated cells in HAE provides
			a useful in vitro model of human lung origin
			to study characteristics of SARS-CoV replication
			and pathogenesis.

			DOI: 10.1128/JVI.79.24.15511-15524.2005
			PMCID: PMC1316022 PMID: 16306622 [Indexed
			for MEDLINE]

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