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			PubMed Journals: Clin Chem

  Source:		PMID: 16195357
  Download:	https://watermark.silverchair.com/clinchem2333.pdf

    		Clin Chem. 2005 Dec;51(12):2333-40. Epub
     		2005 Sep 29.

			Early enhanced expression of interferon-inducible
			protein-10 (CXCL-10) and other chemokines
			predicts adverse outcome in
			severe acute respiratory syndrome.

			Tang NL(1), Chan PK, Wong CK, To KF, Wu
			AK, Sung YM, Hui DS, Sung JJ, Lam CW.

			Author Information
			(1) Department of Chemical Pathology, Faculty
			of Medicine, The Chinese University of Hong Kong,
			Shatin. nelsontang@cuhk.edu.hk

			BACKGROUND:
			Exaggerated activation of cytokines/chemokines has
			been proposed as a factor in adverse outcome of
			severe acute respiratory syndrome (SARS). Previous
			studies on chemokines have included only small
			numbers of patients, and the utility of plasma
			chemokines as prognostic indicators is unclear.
			METHODS: We studied 255 archival plasma samples
			collected during the first or second week after
			disease onset. The chemokines interferon-inducible
			protein-10 (IP-10), monokine induced by
			interferon-gamma (MIG), interleukin-8 (IL-8),
			monocyte chemoattractant protein-1 (MCP-1),
			and regulated upon activation normal T cell
			expressed and secreted (RANTES) were measured
			by cytometric bead array with a 4-color
			FACSCalibur flow cytometer. Reverse transcription
			and real-time quantitative PCR and
			immunohistochemical staining were performed
			to analyze the production of IP-10 in lung
			tissue at autopsy. Conditional logistic
			regression was used to identify independent
			predictors for adverse disease outcome.
			RESULTS: Increases in IP-10, MIG, and IL-8
			during the first week after onset of fever
			were associated with adverse outcome (intensive
			care unit admission or death) in the univariate
			analysis. During the second week, only MIG
			concentration was associated with prognosis.
			After adjusting for other risk factors,
			plasma IP-10 concentration at the first
			week remained as an independent prognostic
			factor, with an odds ratio for adverse outcome
			of 1.52 (95% confidence interval, 1.05-2.55)
			per fold increase in plasma IP-10 concentration
			above the median. During the second week,
			chemokines provided little independent prognostic
			information. IP-10 was increased in lung tissue
			from patients who died of SARS. CONCLUSIONS:
			Increased plasma IP-10 during the first week of
			SARS symptoms is an independent predictor of
			outcome. Chemokine activation may be an early
			event in SARS, and an exaggerated host
			response may produce complications.

			DOI: 10.1373/clinchem.2005.054460 PMID: 16195357
			[Indexed for MEDLINE]

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