PubMed Journals: Dev Cell

  Source:		PMID: 16139227

    		Dev Cell. 2005 Sep;9(3):389-402.
			Akt/PKB regulates actin organization and
			cell motility via Girdin/APE.

			Enomoto A(1), Murakami H, Asai N, Morone
			N, Watanabe T, Kawai K, Murakumo Y, Usukura
			J, Kaibuchi K, Takahashi M.

			Author Information
			(1) Department of Pathology, Center for
			Neurological Disease and Cancer, Nagoya University
			Graduate School of Medicine, Showa-ku, Aichi,

			The serine/threonine kinase Akt (also called
			protein kinase B) is well known as an important
			regulator of cell survival and growth and
			has also been shown to be required for cell
			migration in different organisms. However,
			the mechanism by which Akt functions to
			promote cell migration is not understood.
			Here, we identify an Akt substrate, designated
			Girdin/APE (Akt-phosphorylation enhancer),
			which is an actin binding protein. Girdin
			expresses ubiquitously and plays a crucial
			role in the formation of stress fibers and
			lamellipodia. Akt phosphorylates serine
			at position 1416 in Girdin, and phosphorylated
			Girdin accumulates at the leading edge of
			migrating cells. Cells expressing mutant
			Girdin, in which serine 1416 was replaced
			with alanine, formed abnormal elongated
			shapes and exhibited limited migration and
			lamellipodia formation. These findings suggest
			that Girdin is essential for the integrity
			of the actin cytoskeleton and cell migration
			and provide a direct link between Akt and
			cell motility.

			DOI: 10.1016/j.devcel.2005.08.001 PMID: 16139227
			[Indexed for MEDLINE]

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