PubMed Journals: J Pathol

  Source:		PMID: 14743497
  Download:	https://onlinelibrary.wiley.com/doi/epdf/10.1002/path.1510

    		J Pathol. 2004 Feb;202(2):157-63.
			Tissue and cellular tropism of the coronavirus
			associated with severe acute respiratory syndrome:
			an in-situ hybridization study of fatal cases.

			To KF(1), Tong JH, Chan PK, Au FW, Chim
			SS, Chan KC, Cheung JL, Liu EY, Tse GM,
			Lo AW, Lo YM, Ng HK.

			Author Information
			(1) Department of Anatomical and Cellular
			Pathology, The Chinese University of Hong Kong,
			Hong Kong SAR, China. kfto@cuhk.edu.hk

			Comment in J Pathol. 2004 Jun;203(2):729-30;
			author reply 730-1.

			Severe acute respiratory syndrome (SARS)
			is a new human infectious disease with significant
			morbidity and mortality. The disease has
			been shown to be associated with a new coronavirus
			(SARS-CoV). The clinical and epidemiological
			aspects of SARS have been described. Moreover,
			the viral genome of SARS-CoV has been fully
			sequenced. However, much of the biological
			behaviour of the virus is not known and
			data on the tissue and cellular tropism
			of SARS-CoV are limited. In this study,
			six fatal cases of SARS were investigated
			for the tissue and cellular tropism of SARS-CoV
			using an in-situ hybridization (ISH) technique.
			Among all the tissues studied, positive
			signals were seen in pneumocytes in the
			lungs and surface enterocytes in the small
			bowel. Infected pneumocytes were further
			confirmed by immunofluorescence-fluorescence
			in-situ hybridization (FISH) analysis. These
			results provide important information concerning
			the tissue tropism of SARS-CoV, which is
			distinct from previously identified human
			coronaviruses, and suggest the possible
			involvement of novel receptors in this infection.
			Whereas the lung pathology was dominated
			by diffuse alveolar damage, the gut was
			relatively intact. These findings indicated
			that tissue responses to SARS-CoV infection
			are distinct in different organs.

			Copyright 2004 John Wiley & Sons, Ltd.

			DOI: 10.1002/path.1510 PMID: 14743497
			[Indexed for MEDLINE]

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