PubMed Journals: J Pathol
Source: PMID: 14743497
⇦ ⇨ J Pathol. 2004 Feb;202(2):157-63.
Tissue and cellular tropism of the coronavirus
associated with severe acute respiratory syndrome:
an in-situ hybridization study of fatal cases.
To KF(1), Tong JH, Chan PK, Au FW, Chim
SS, Chan KC, Cheung JL, Liu EY, Tse GM,
Lo AW, Lo YM, Ng HK.
(1) Department of Anatomical and Cellular
Pathology, The Chinese University of Hong Kong,
Hong Kong SAR, China. firstname.lastname@example.org
Comment in J Pathol. 2004 Jun;203(2):729-30;
author reply 730-1.
Severe acute respiratory syndrome (SARS)
is a new human infectious disease with significant
morbidity and mortality. The disease has
been shown to be associated with a new coronavirus
(SARS-CoV). The clinical and epidemiological
aspects of SARS have been described. Moreover,
the viral genome of SARS-CoV has been fully
sequenced. However, much of the biological
behaviour of the virus is not known and
data on the tissue and cellular tropism
of SARS-CoV are limited. In this study,
six fatal cases of SARS were investigated
for the tissue and cellular tropism of SARS-CoV
using an in-situ hybridization (ISH) technique.
Among all the tissues studied, positive
signals were seen in pneumocytes in the
lungs and surface enterocytes in the small
bowel. Infected pneumocytes were further
confirmed by immunofluorescence-fluorescence
in-situ hybridization (FISH) analysis. These
results provide important information concerning
the tissue tropism of SARS-CoV, which is
distinct from previously identified human
coronaviruses, and suggest the possible
involvement of novel receptors in this infection.
Whereas the lung pathology was dominated
by diffuse alveolar damage, the gut was
relatively intact. These findings indicated
that tissue responses to SARS-CoV infection
are distinct in different organs.
Copyright 2004 John Wiley & Sons, Ltd.
DOI: 10.1002/path.1510 PMID: 14743497
[Indexed for MEDLINE]