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			PubMed Journals: N Engl J Med

  Source:		PMID: 12690091
  Download:	https://www.nejm.org/doi/pdf/10.1056/NEJMoa030747

    		N Engl J Med. 2003 May 15;348(20):1967-76.
     		Epub 2003 Apr 10.

			Identification of a novel coronavirus in
			patients with severe acute respiratory syndrome.

			Drosten C(1), Günther S, Preiser W, van
			der Werf S, Brodt HR, Becker S, Rabenau
			H, Panning M, Kolesnikova L, Fouchier RA,
			Berger A, Burguière AM, Cinatl J, Eickmann
			M, Escriou N, Grywna K, Kramme S, Manuguerra
			JC, Müller S, Rickerts V, Stürmer M, Vieth
			S, Klenk HD, Osterhaus AD, Schmitz H, Doerr
			HW.

			Author Information
			(1) Bernhard Nocht Institute for Tropical Medicine,
			National Reference Center for Tropical Infectious
			Diseases, Hamburg, Germany. drosten@bni-hamburg.de

			Comment in N Engl J Med. 2003 May
			15;348(20):1948-51. N Engl J Med. 2003 May
			15;348(20):1947-8.

			BACKGROUND: The severe acute respiratory syndrome
			(SARS) has recently been identified
			as a new clinical entity. SARS is thought
			to be caused by an unknown infectious agent.
			METHODS: Clinical specimens from patients
			with SARS were searched for unknown viruses
			with the use of cell cultures and molecular
			techniques. RESULTS: A novel coronavirus
			was identified in patients with SARS. The
			virus was isolated in cell culture, and
			a sequence 300 nucleotides in length was
			obtained by a polymerase-chain-reaction
			(PCR)-based random-amplification procedure.
			Genetic characterization indicated that
			the virus is only distantly related to known
			coronaviruses (identical in 50 to 60 percent
			of the nucleotide sequence). On the basis
			of the obtained sequence, conventional and
			real-time PCR assays for specific and sensitive
			detection of the novel virus were established.
			Virus was detected in a variety of clinical
			specimens from patients with SARS but not
			in controls. High concentrations of viral
			RNA of up to 100 million molecules per milliliter
			were found in sputum. Viral RNA was also
			detected at extremely low concentrations
			in plasma during the acute phase and in
			feces during the late convalescent phase.
			Infected patients showed seroconversion
			on the Vero cells in which the virus was
			isolated. CONCLUSIONS: The novel coronavirus
			might have a role in causing SARS.

			Copyright 2003 Massachusetts Medical Society

			DOI: 10.1056/NEJMoa030747 PMID: 12690091
			[Indexed for MEDLINE]

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