PubMed Journals: Int J Mol Med
Source: PMID: 12119563
⇦ ⇨ Int J Mol Med. 2002 Aug;10(2):221-5.
Molecular cloning and characterization of
OSR1 on human chromosome 2p24.
(1) Genetics and Cell Biology Section, Genetics
Division, National Cancer Center Research
Institute, Tsukiji 5-chome, Chuo-ku, Tokyo
104-0045, Japan. email@example.com
During Drosophila hindgut development, bowl,
fork head/FOX, drumstick, lines, and wingless/WNT
play important roles. Drosophila bowl gene
is homologous to Drosophila odd-skipped
(odd) gene and odd-skipped related gene
(sob). Here, human OSR1, related to Drosophila
odd, was isolated using bioinformatics and
cDNA-PCR. OSR1 was found to encode 266 amino-acid
protein with three C2H2-type zinc fingers,
a tyrosine phosphorylation site (Tyr 203),
and several putative PXXP SH3 binding motifs.
Three zinc fingers and a tyrosine phosphorylation
site were conserved among human OSR1, OSR2,
Drosophila odd, sob, and bowl. OSR1 showed
63.6% total amino-acid identity with OSR2.
OSR1 gene consisting of three exons was
located on human chromosome 2p24. OSR1 mRNA
of 2.3-kb in size was detected in adult
colon, small intestine, prostate, testis,
and fetal lung. OSR1 mRNA was significantly
up-regulated in a pancreatic cancer cell
line MIA PaCa-2, and was weakly expressed
in gastric cancer cell lines OKAJIMA, MKN45,
pancreatic cancer cell lines PANC-1, BxPC-3,
AsPC-1, PSN-1, Hs766T, and esophageal cancer
cell line TE10. Among 10 cases of primary
gastric cancer, OSR1 mRNA was up-regulated
in 5 cases, and was down-regulated in 2
cases. This is the first report on molecular
cloning and characterization of human OSR1.
PMID: 12119563 [Indexed for MEDLINE]