PubMed Journals: Int J Mol Med

  Source:		PMID: 12119563

    		Int J Mol Med. 2002 Aug;10(2):221-5.
			Molecular cloning and characterization of
			OSR1 on human chromosome 2p24.

			Katoh M(1).

			Author Information
			(1) Genetics and Cell Biology Section, Genetics
			Division, National Cancer Center Research
			Institute, Tsukiji 5-chome, Chuo-ku, Tokyo
			104-0045, Japan. mkatoh@ncc.go.jp

			During Drosophila hindgut development, bowl,
			caudal/CDX, brachyenteron/Brachyury/TBX,
			fork head/FOX, drumstick, lines, and wingless/WNT
			play important roles. Drosophila bowl gene
			is homologous to Drosophila odd-skipped
			(odd) gene and odd-skipped related gene
			(sob). Here, human OSR1, related to Drosophila
			odd, was isolated using bioinformatics and
			cDNA-PCR. OSR1 was found to encode 266 amino-acid
			protein with three C2H2-type zinc fingers,
			a tyrosine phosphorylation site (Tyr 203),
			and several putative PXXP SH3 binding motifs.
			Three zinc fingers and a tyrosine phosphorylation
			site were conserved among human OSR1, OSR2,
			Drosophila odd, sob, and bowl. OSR1 showed
			63.6% total amino-acid identity with OSR2.
			OSR1 gene consisting of three exons was
			located on human chromosome 2p24. OSR1 mRNA
			of 2.3-kb in size was detected in adult
			colon, small intestine, prostate, testis,
			and fetal lung. OSR1 mRNA was significantly
			up-regulated in a pancreatic cancer cell
			line MIA PaCa-2, and was weakly expressed
			in gastric cancer cell lines OKAJIMA, MKN45,
			pancreatic cancer cell lines PANC-1, BxPC-3,
			AsPC-1, PSN-1, Hs766T, and esophageal cancer
			cell line TE10. Among 10 cases of primary
			gastric cancer, OSR1 mRNA was up-regulated
			in 5 cases, and was down-regulated in 2
			cases. This is the first report on molecular
			cloning and characterization of human OSR1.

			PMID: 12119563 [Indexed for MEDLINE]

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