PubMed Journals: Nat Cell Biol

  Source:		PMID: 11331870

    		Nat Cell Biol. 2001 May;3(5):439-44.
			Regulation of the PH-domain-containing tyrosine
			kinase Etk by focal adhesion kinase through
			the FERM domain.

			Chen R(1), Kim O, Li M, Xiong X, Guan JL,
			Kung HJ, Chen H, Shimizu Y, Qiu Y.

			Author Information
			(1) Departments of Laboratory Medicine and
			Pathology and Pharmacology, University of
			Minnesota, Minneapolis, Minnesota 55455,

			Etk/BMX, a member of the Btk family of tyrosine
			kinases, is highly expressed in cells with
			great migratory potential, including endothelial
			cells and metastatic carcinoma cell lines.
			Here, we present evidence that Etk is involved
			in integrin signalling and promotes cell
			migration. The activation of Etk by extracellular
			matrix proteins is regulated by FAK through
			an interaction between the PH domain of
			Etk and the FERM domain of FAK. The lack
			of Etk activation by extracellular matrix
			in FAK-null cells could be restored by
			co-transfection with wild-type FAK. Disrupting
			the interaction between Etk and FAK diminished
			the cell migration promoted by either kinase.
			Furthermore, inhibiting Etk expression in
			metastatic carcinoma cell lines with an
			antisense oligonucleotide blocks integrin-mediated
			migration of these cells. Taken together,
			our data indicate the essential role of
			the interaction of the PH domain of Etk
			and the FERM domain of FAK in integrin signalling.

			DOI: 10.1038/35074500 PMID: 11331870 [Indexed
			for MEDLINE]

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