PubMed Journals: J Neurosci

  Source:		PMID: 10844023

    		J Neurosci. 2000 Jun 15;20(12):4535-44.
			Phosphorylated syntaxin 1 is localized to discrete
			domains along a subset of axons.

			Foletti DL(1), Lin R, Finley MA, Scheller

			Author Information
			(1) Howard Hughes Medical Institute, Department
			of Molecular and Cellular Physiology,
			Stanford University School of Medicine,
			Stanford, California 94305-5428, USA.

			Syntaxin 1 is a SNARE protein that plays a
			central role in synaptic vesicle (SV) exocytosis.
			We generated an antibody that specifically
			recognizes a casein kinase II-mediated
			phosphorylation on serine-14 of syntaxin
			1. In this report we show that this phosphorylation
			occurs in vivo and is developmentally regulated
			in the rat brain, rising to a level of 40%
			of the total syntaxin in adult animals.
			Phosphorylated syntaxin is preferentially
			associated with SNAP-25 and localizes to
			discrete domains of the axonal plasma membrane
			that do not colocalize with pools of synaptic
			vesicles. These phosphosyntaxin domains
			may define fusion sites for a novel class
			of vesicles outside classical active zones.

			PMID: 10844023 [Indexed for MEDLINE]

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