PubMed Journals: J Neurosci
Source: PMID: 10844023
⇦ ⇨ J Neurosci. 2000 Jun 15;20(12):4535-44.
Phosphorylated syntaxin 1 is localized to discrete
domains along a subset of axons.
Foletti DL(1), Lin R, Finley MA, Scheller
(1) Howard Hughes Medical Institute, Department
of Molecular and Cellular Physiology,
Stanford University School of Medicine,
Stanford, California 94305-5428, USA.
Syntaxin 1 is a SNARE protein that plays a
central role in synaptic vesicle (SV) exocytosis.
We generated an antibody that specifically
recognizes a casein kinase II-mediated
phosphorylation on serine-14 of syntaxin
1. In this report we show that this phosphorylation
occurs in vivo and is developmentally regulated
in the rat brain, rising to a level of 40%
of the total syntaxin in adult animals.
Phosphorylated syntaxin is preferentially
associated with SNAP-25 and localizes to
discrete domains of the axonal plasma membrane
that do not colocalize with pools of synaptic
vesicles. These phosphosyntaxin domains
may define fusion sites for a novel class
of vesicles outside classical active zones.
PMID: 10844023 [Indexed for MEDLINE]